The N-STORM uses stochastic illumination approach to illuminate only a sub-set of the total fluorophores and blink them on and off across thousands of frames
By fitting points to the fluorophore peaks, it can reconstruct an image with a resolution of up to 20 nm in X and Y and 50 nm in Z.
Specifications
The STORM microscope can obtain a resolution of 20 nm in x/y inside a fully hydrated environment. This allows imaging of cells or materials under conditions that are as natural as possible.
- Lasers: 405 nm, 488 nm, 561 nm and 647 nm
- Camera: Andor EMCCD iXon3
- Resolution: 20 nm in x/y, 50 nm in z
Additional capabilities:
- TIRF
- Single-molecule imaging
Access
The NanoSight is available through the MCFP's iLab Booking System for trained users. To discuss access and training please contact Darryl below.
Sample preparation
Sample preparation is very important for super-resolution experiments and is very sample specific, please contact Darryl below.
Additional resources
Darryl Johnson
Academic Specialist - Cytometry
Darryl leads the Cytometry node of the MCFP with expertise in mass and flow cytometry, and confocal microscopy. He has a background in molecular biology, biochemistry and immunology, and can help you with experimental design, preparation, acquisition, and interpretation.
See Also
-
Nikon A1R+ Confocal Microscope (CLSM)
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and large incubation chamber, the Nikon A1R+ is a powerful tool for the imaging and visualisation of intracellular dynamics and interaction
-
Olympus IX71 Fluorescence Microscope
The Olympus IX71 is an inverted widefield microscope fitted with a sensitive camera and a broad range of objectives
-
NanoSight Pro
The Malvern NanoSight Pro instrument provides an easy-to-use, reproducible platform for nanoparticle characterisation