STORM (Stochastic optical reconstruction microscopy): Nikon N-STORM

The N-STORM uses stochastic illumination approach to illuminate only a sub-set of the total fluorophores and blink them on and off across thousands of frames.

By fitting points to the fluorophore peaks, it can reconstruct an image with a resolution of up to 20 nm in X and Y and 50 nm in Z.


The STORM microscope can obtain a resolution of 20 nm in x/y inside a fully hydrated environment. This allows imaging of cells or materials under conditions that are as natural as possible.

  • Lasers: 405 nm, 488 nm, 561 nm and 647 nm
  • Camera: Andor EMCCD iXon3
  • Resolution: 20 nm in x/y, 50 nm in z

Additional capabilities:

  • TIRF
  • Single-molecule imaging


The NanoSight is available through the MCFP's iLab Booking System for trained users. To discuss access and training please contact Darryl Johnson.

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Sample preparation

Sample preparation is very important for super-resolution experiments and is very sample specific, please contact Darryl Johnson.

Additional resources

Company website