The Elyra is a fast and flexible super-resolution microscope for live cell imaging beyond the diffraction limit
Specifications
The Zeiss Elyra 7 is a super-resolution Lattice SIM system capable of high speed 3D live cell imaging at twice the resolution of conventional microscopy, vastly improving acquisition time over standard SIM. And for appropriate fixed samples, single molecule imaging allows resolution of objects as small as 20nm, all on the same instrument.
- Lasers: 405nm, 488nm, 561nm and 642nm
- Cameras: 2x PCO.EDGE 4.2 CLHS sCMOS
- Resolution: 110nm lateral (Lattice SIM), 20nm lateral (STORM/PALM)
Additional capabilities:
- TIRF
- 3D single-molecule imaging
Access
The Zeiss Elyra 7 is available through the MCFP's iLab Booking System for trained users. To discuss access and training please contact Paul Brannon or Darryl Johnson.
Sample preparation
Sample preparation for Lattice SIM is similar to confocal microscopy, compatible with most commonly used dyes with no additional steps. For single molecule imaging (STORM/PALM), sample-specific protocols are often necessary. Please contact Paul Brannon or Darryl Johnson for details.
Additional resources
See Also
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Nikon A1R+ Confocal Microscope (CLSM)
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and large incubation chamber, the Nikon A1R+ is a powerful tool for the imaging and visualisation of intracellular dynamics and interaction.
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STORM (Stochastic optical reconstruction microscopy): Nikon N-STORM
The N-STORM uses stochastic illumination approach to illuminate only a sub-set of the total fluorophores and blink them on and off across thousands of frames.
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NanoSight Pro
The Malvern NanoSight Pro instrument provides an easy-to-use, reproducible platform for nanoparticle characterisation.