Zeiss Elyra 7 Lattice SIM

The Elyra is a fast and flexible super-resolution microscope for live cell imaging beyond the diffraction limit

Specifications

The Zeiss Elyra 7 is a super-resolution Lattice SIM system capable of high speed 3D live cell imaging at twice the resolution of conventional microscopy, vastly improving acquisition time over standard SIM. And for appropriate fixed samples, single molecule imaging allows resolution of objects as small as 20nm, all on the same instrument.

  • Lasers: 405nm, 488nm, 561nm and 642nm
  • Cameras: 2x PCO.EDGE 4.2 CLHS sCMOS
  • Resolution: 110nm lateral (Lattice SIM), 20nm lateral (STORM/PALM)

Additional capabilities:

  • TIRF
  • 3D single-molecule imaging

Access

The Zeiss Elyra 7 is available through the MCFP's iLab Booking System for trained users. To discuss access and training please contact Darryl below.

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Sample preparation

Sample preparation for Lattice SIM is similar to confocal microscopy, compatible with most commonly used dyes with no additional steps. For single molecule imaging (STORM/PALM), sample-specific protocols are often necessary. Please contact Darryl below for details.

Additional resources

Zeiss product page

Darryl Johnson

Darryl Johnson

Academic Specialist - Cytometry

Darryl leads the Cytometry node of the MCFP with expertise in mass and flow cytometry, and confocal microscopy. He has a background in molecular biology, biochemistry and immunology, and can help you with experimental design, preparation, acquisition, and interpretation.

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