The Amnis Imagestream X MkII Imaging Flow Cytometer (ISX) bridges the gap between microscopy and flow cytometry by combining the structural information from microscopy and the high throughput nature of flow cytometry.
The Amnis Imagestream X MkII Imaging Flow Cytometer (ISX) bridges the gap between microscopy and flow cytometry by combining the structural information of microscopy with the high throughput nature of flow cytometry.
The ISX can image 12 channels simultaneously, including 10 fluorescence plus brightfield and side scatter at up to 5000 cells per second. This combination of speed, imaging information and sensitivity make it suitable for a wide range of experiments, including identifying colocalisation, internalisation, nuclear fragmentation and cell morphology.
Specifications
Manufacturer: Amnis, Imagestream X MkII
- 4 lasers (405 nm, 488 nm, 561 nm, 642 nm)
- 12 channels (10 fluorescence)
- Multi-mag capability (×20, ×40, ×60)
- Extended Depth of Field module
- Autosampler
- Up to 5,000 cells per second
Access
The Apogee is available on the MCFP's iLab Booking System. For new users to this instrument, please contact either Paul Brannon or Darryl Johnson to arrange training.
Sample Preparation
If you would like assistance with sample preparation please contact either Paul Brannon or Darryl Johnson
Additional Resources
See Also
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Apogee A-50 Microflow Cytometer
The Apogee A-50 Microflow Cytometer is a benchtop flow cytometer with extreme sensitivity and resolution from multiple light scatter and fluorescence detectors.
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NanoSight Pro
The Malvern NanoSight Pro instrument provides an easy-to-use, reproducible platform for nanoparticle characterisation.
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Nikon A1R+ Confocal Microscope (CLSM)
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and large incubation chamber, the Nikon A1R+ is a powerful tool for the imaging and visualisation of intracellular dynamics and interaction.