Imaging Flow Cytometry

The Amnis Imagestream X MkII Imaging Flow Cytometer (ISX) bridges the gap between microscopy and flow cytometry by combining the structural information from microscopy and the high throughput nature of flow cytometry

The Amnis Imagestream X MkII Imaging Flow Cytometer (ISX) bridges the gap between microscopy and flow cytometry by combining the structural information of microscopy with the high throughput nature of flow cytometry.

The ISX can image 12 channels simultaneously, including 10 fluorescence plus brightfield and side scatter at up to 5000 cells per second. This combination of speed, imaging information and sensitivity make it suitable for a wide range of experiments, including identifying colocalisation, internalisation, nuclear fragmentation and cell morphology.

Specifications

Manufacturer: Amnis, Imagestream X MkII

  • 4 lasers (405 nm, 488 nm, 561 nm, 642 nm)
  • 12 channels (10 fluorescence)
  • Multi-mag capability (×20, ×40, ×60)
  • Extended Depth of Field module
  • Autosampler
  • Up to 5,000 cells per second

Access

The Apogee is available on the MCFP's iLab Booking System. For new users to this instrument, please contact either Darryl below to arrange training.

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Sample Preparation

If you would like assistance with sample preparation please contact either Darryl below.

Additional Resources

Website

Darryl Johnson

Darryl Johnson

Academic Specialist - Cytometry

Darryl leads the Cytometry node of the MCFP with expertise in mass and flow cytometry, and confocal microscopy. He has a background in molecular biology, biochemistry and immunology, and can help you with experimental design, preparation, acquisition, and interpretation.

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